Description
For Sample For the qualitative determination of human rheumatoid factor (RF) antibody (IgM) concentrations in serum, plasma.
PRINCIPLE OF THE ASSAY This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgM conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human rheumatoid factor (RF) antibody (IgM) bound in the initial step. The color development is stopped and the intensity of the color is measured